Pair Name	Fisetin, Fluorouracil
Disease Info	[ICD-11: 2B91.Z]	Colorectal cancer		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Down-regulation	Expression	PIK3CA	hsa5290
	Down-regulation	Phosphorylation	AKT1	hsa207
	Down-regulation	Phosphorylation	MTOR	hsa2475
	Up-regulation	Phosphorylation	PRKAA1	hsa5562
	Down-regulation	Phosphorylation	EIF4EBP1	hsa1978
	Down-regulation	Phosphorylation	EIF4E	KEGG ID N.A.
	Down-regulation	Phosphorylation	RPS6KB1	hsa6198
	Down-regulation	Expression	RPTOR	hsa57521
	Down-regulation	Expression	RICTOR	hsa253260
	Down-regulation	Expression	MLST8	KEGG ID N.A.
	Down-regulation	Phosphorylation	AKT1S1	hsa84335
In Vitro Model	SW480	Colon adenocarcinoma	Homo sapiens (Human)	CVCL_0546
	HCT 116	Colon carcinoma	Homo sapiens (Human)	CVCL_0291
	HT-29	Colon adenocarcinoma	Homo sapiens (Human)	CVCL_0320
In Vivo Model	Since FC13K1ApcMin/+ mice develop adenomas around 1 month of age, the treatment with fisetin and 5-FU by intraperitoneal injection was started between 26 and 30 days.
Result	Fisetin and 5-fluorouracil: Effective combination for PIK3CA-mutant colorectal cancer